Nano silver is used in growing and preserving decorative flowers for Tet holidays

Nano silver to preserve lay flowers on New Year's Day

(Copyright by NanoCMM Technology)

Nanotechnology, which is one of the most promising scientific fields, offers limitless possibilities of developing many fields, including agriculture and horticulture. Silver nanomaterials are one of the nanomaterials whose effects on plants are being studied today. Numerous experiments conducted by scientists have evaluated the use of silver nanoparticles as a regulator for plants, and also as a means to prolong the post-harvest life of cut flowers and foliage. mind. This paper summarizes a comprehensive and systematic study of data on nano silver activity and its application in horticulture, post-harvest treatment and preservation of cut flowers and semi-flowering plants.

 

Nano silver characteristics
Research in the nanotechnology field is highly prioritized and covers disciplines such as chemistry, physics, biology, materials engineering, agriculture, and more.
Nanotechnology consists of a set of techniques and methods for creating structures with particle sizes ranging from 1 nm to 100 nm [1,2]. In recent years, we can observe a lot in metal nanoparticles, both because of their unlimited applicability, as well as the unusual biological, chemical and physical properties of metal nanoparticles. they.
Silver is one of the earliest discovered precious metals and its specialty
The quality was known in 4000 BC [4]. Silver’s bactericidal function is known since ancient times. Its medicinal properties have been described by Hippocrates (in the fourth century BC) [5,6]. Silver ions have long found their uses in medicine and medicine, veterinary medicine and the food industry, and are used as protection against
bacteria, fungi and viruses that cause disease [6,7]. Due to the fragmented particles in nanoscale, silver possesses new properties that differ significantly from its macroscopic structure and structure of the same chemical compound [8]. It is a consequence of the presence of a large number of atoms located on the surface of the particle, having an exposure to the outside environment [9]. Silver nanoparticles are characterized by large
surface area relative to the micrometer form, has a significant effect on the absorption of bactericidal properties and activity against bacteria [10]. Besides silver’s specific surface nanoparticles are also characterized by large surface atomic fractions and having unique physical and chemical properties.

 

Mechanism of action of nano silver
Nanoparticles are characterized by much higher biochemical activity than macroscopic structures and therefore affect more effectively on bacteria or fungi [5,6,12]. Silver’s nanoparticles have a specific ability to oxidize the substances that surround it. A silver nanoparticle is characterized by a high electrical charge and its shell does not have two electrons, which attracts pathogenic microorganisms. By intercepting the missing electrons, the silver nanoparticles injure and then destroy the pathogen. This mechanism gives the nanoparticles a strong bactericidal and fungicidal ability and has certain antiviral properties. The mechanism of the nano-effects on fungi and viruses is similar to the effect on bacterial cells. Silver nano disrupts the water balance of the fungus and affects the catalytic breakdown of the lipid-protein layers of the virus [4]. It has been shown that the bactericidal activity of the nanoparticles is dependent on the size and shape of the flea colonies, however, it is important to emphasize that the mechanism of microbial inactivation has not yet been achieved. correctly explained so far [13,14].
The effectiveness of nano silver in cultivation
This review collects information on nano silver provided by recent research, especially when used in horticulture. The application of nano silver increases plant height [15,16,18], number of leaves [16], root weight [19,20], plant biomass [15,16,25,38], sprout seeds [19,20,25,28,36,37], seed yield [15,16], fruit quality [18], stem length [22,30], canopy area diameter [21] , root length [19,20, 22,24,25,27,30,32,35-37], growth and development of explants under in vitro culture conditions [31] and number of platelets [30]. The appropriate addition of silver nanoparticles increased chlorophyll content [27], carotenoid [22], flavonoid [31], photosynthetic quantum efficiency [27], enzyme activity, parahydroxy benzoic acid content. [17], α-terpinyl acetate [21], as well as the antioxidant enzyme activity [29]. Experimental results on the use of silver nanoparticles in plants are summarized in Table 1.

 

Table 1 The growth stimulating effects of silver nanoparticles in plants

PlantNano silver concentrationMethod of applicationEffectiveReference
Basil

Ocimum basilicum )

 

(20, 40 and 60 ppm)Spray at seed development stage
● Increase plant height
● Improve the dry weight of plants
● Improve seed yield
[15]

 

Borage

Borago officinalis )

 

(20, 40 and 60

ppm)

 

Spray on plants 125 days after planting
● Improve seed yield
● Increase the number of leaves
● Raise plant height
● Improve the dry weight of plants
● Increase the dry weight of the inflorescence
[16]

 

Castor

Ricinus communis )

 

 (100, 200, 500, 1000, 2000, 4000 ppm )Soak the seeds
● Increased enzyme activity
● Advanced content of parahydroxy benzoic acid
[17]

 

Cucumber

Cucumis

sativus )

 

(average size 50 nm, bulk density 0.92 g.ml-1, specific surface area 10.1 m2.g-1) at 500, 1000, 1500, 2000, 2500 and 3000 ppm)Spray every 7 days for 14 weeks
● Increase plant height
● Improve the amount of fruit
● Increase fruit weight
● Increase the length of the left
[18]

 

Dill

(Foeniculum vulgare)

(0, 20, 40, 60, 80, 100 ppm and 0, 30, 60, 90,120,150,180 ppm)Add to the in vitro medium
● Increase the germination rate
● Improve original fresh weight
● Advanced root length
[19]

 

Alfalfa

Trigonella foenum

graecum )

 

(0, 10, 20, 30, 40 ppm )Soak seeds (15 ml for 12 days)
● Increase root length
● Improve original fresh weight
● Increase the dry root weight
● Increase seed germination
[20]
Ferula rigidula (Thymus kotschyanus)(0, 20, 40, 60, 80 and 100 pm)Soak the seeds
● Increase the diameter of the canopy area
● The flowering time is shortened
● Refined essential oils
● Increase the yield of herbs
● Increase the content of α-terpinyl acetate
[21]
Sugar beet

(Beta vulgaris)

(0,5, 15 ppm or 0,5, 15 mg / kg)Add to environment and soil
● Advanced root length
● Increase stem length
● Improved carotenoid content
[22]
Rye grass

(Lolium multiflorum)

(1, 5, 10, 20, 40 ppm )Soak seeds for 1 hour● Inhibits seedling growth[23]
Hibiscus

(Hibiscus rosa sinensis)

Add the IAA and BA
● Advanced root length
● Increase the number of roots
[24]
Lentils

(Lens culinaris)

(10, 20, 30 and 40 ppm)Add to seeds (15 ml for 14 days)
● Increase root length
● Improve shooting length
● Dry volume increases
● Enhance seed germination
[25]
Green bean

(Phaolus radiatus)

(5, 10, 20, 40 ppm and 500, 1000, 2000 ppm)Add to the agar and soil medium● Inhibits seedling growth[26]
Broccoli

(Brassica juncea)

(25, 50, 100, 200 and 400 ppm)Add to the in vitro medium
● Increase original length
● Increased chlorophyll content
● Improved photosynthesis quantum efficiency
[27]
Pearl millet

(Pennisetum glaucum)

 (20 and 50 ppm )Soak the seeds for 2 hours
● Increase seed germination
● Improve shooting length
● Advanced root length
[28]
Thien Truc kneeling
Or African Geranium

(Pelargonium zonale)

(0, 20, 40, 60, and 80 ppm )Spray 50 mL
● Increased antioxidant enzyme activity
● Reducing lipid peroxidation
● Improve the lifespan of petals
● Reduce the pressure of the petals
[29]
Potato

(Solanum tuberosum)

(0, 1.0, 1.5, and 2.0 ppm)Add to the in vitro medium
● Increase stem length
● Improved root length
● Reduces the number of isolated platelets
● The viability of isolates is reduced
[30]
Potato ‘White Desiree’

(Solanum tuberosum)

(average size 20 nm, sphere and specific surface
area 18-22 m2 / g at 0, 2, 10, 20 ppm)
Add to the in vitro medium
● Increase flavonoid content
● Total phenol is enhanced
● Improved culture growth and development under in vitro culture conditions
[31]
Radish

(Raphanus sativus)

(125, 250, and 500 ppm )Add to the in vitro medium
● Reduced water content
● Reduced root length
[32]
Rose

(Rosa hybrida)

(0, 50, 100 và 150 ppm)Add to the in vitro medium
● Reduce bacterial contamination
● Reduce the rate of phenol secretion
[33]
Safflower tree

(Carthamus tinctorius)

(20, 40, 60 ppm)Soak the seeds
● Increase the number of seeds to sprout
● Improve the number of flower clusters
[34]
Saffron

(Crocus sativus)

(0, 40, 80 or 120 ppm)Soak the stem for 90 minutes
● Increase the number of roots
● Improved root length
● Increase the weight of dry leaves
[35]
Tomato

(Lycopersicon esculentum)

(0, 25, 50, 75 and 100 ppm)
Soak the seeds
(5 seconds, three times in 1 hour)
● Increase germination index in the early stage
● Reduced root length
● The length of the bud is reduced
[36]
Tomato

(Solanum lycopersicum)

(0.05, 0.5, 1.5, 2, 2.5 ppm )Soak seeds for 2 hours
● Increase the germination rate
● Improve germination rate
● Advanced root length
● Increase the weight of fresh and dry seedlings
[37]
Mustard greens

(Brassica

juncea)

(50 and 75 ppm)Spray the leaves
● Improve the dry weight of shoots
● Increase the weight of fresh shoots
● Enhanced shooting duration
[38]

 

As demonstrated in many studies [39-41,43,44,46-59], nano-silver can be an effective agent to prolong the post-harvest life of cut flowers. The positive effects of nano-silver on the shelf life of day-expressed cut flowers have been reported for colloid [39], alstroemeria [40], aspidistria [41], carnation [42.43], and chrysanthemum [ 44-46], freesia [47], gerbera [48-50], gladiolus [51], oriental lily [52,53], rose [54-58], lily [59 , 60] and tulips [62]. The study results showed that nano-silver had a positive effect on flower fresh weight [39,41,49,52-54,59,61], stem diameter [59], survival rate and succulence of cut flowers [45], absorption solution [39,41,48,50,51-56,58,59], water balance [58], chlorophyll content [40,60,61], stomatal conductivity [55], open flower cluster [44.47], greenness index [41.46] and electrolyte leakage [60]. Documented data provide evidence that nano silver is very effective in inhibiting the growth of water microbiota [42,45,48,50-53,55], ethylene production [57], as well as bacterial population [42]. The experimental results on the use of silver nanoparticles in cut flowers and greens are summarized in Table 2.
Table 2. Effects of nano-silver on cut flowers and semi-flowering plants.
Plants Nano silver concentrationMethod of applicationEffectiveReference source
Acacia

(Acacia holosericea)

(neutral 4 or 40 mg L-1, acid 0.5 or 5 mg L-1 and ion 0.5 or 1 mg L-1)Pulsing for 24 h
● Increase the shelf life of leaves
● Improve leaf bud mass
● Increase plant solution consumption
[39]
Lily flower

‘Rosada’

(Alstroemeria)

(15, 20 and 25 ppm)Add to the flower arrangement solution
● Increase the life of the vase
● Reduces neck curl
● Improved chlorophyll content
[40]
Aspidistra

(Aspidistra elatior)

(0.01%, 0.02% and 0.04%)Add to the flower arrangement solution
● Increase the laminas blueness index
● Improved fresh weight
● The life of the vase is extended
● Increases relative solution absorption
[41]
Carnation

(Dianthus caryophyllus)

(0, 5, 10 and 15 mg L-1 )Pulsing for 24 h
● Reduces oxidative stress
● Improved antioxidant system
● Decreased bacterial populations
[42]
Carnation

‘Cream Viana’

(Dianthus)

(2 and 4 ppm)Pulsing for 48 h
● The life of the vase is extended
● Increase anthocyanins content
[43]
‘Puma’ chrysanthemum

(Chrysanthemum)

(0.01, 0.05, 0.1, 0.5, 1, and 5 mM)Add to the flower arrangement solution
● Open flower cluster is promoted
● Increase the life of the vase
[44]
Chrysanthemum

(Hoa cúc

morifolium)

(0, 5, 10 and 20 ppm )Pulsing for 24 h
● Increase the viability and succulence of cut flowers
● Reduced stem bacteria colonies
[45]
Chrysanthemum

‘Feeling Green’

(Chrysanthemum)

Colloidal silver (0.01, 0.02 and 0.04%)Add to the flower arrangement solution
● The life of the vase is extended
● Weight loss has been inhibited
● Increase the leaf greenness index
[46]
Orchid Freesia

(Freesia)

Nano silver (5, 10 and 15 ppm)Add to the flower arrangement solution
● Increase the life of the vase
● Improve the number of flowers opened
[47]
Gerbera

(Hoa đồng tiền jamesonii)

(1, 2, 3, 4 and 5 ppm )Add in preservative solutions
● Inhibits the growth of microorganisms in the flask solution
● The life of the vase is extended
● Increase the plant’s liquid consumption
[48]
Gerbera (Gerbera jamesonii)5 hoặc 10 ppmAdd to the flower arrangement solution
● Increase fresh weight
● The life of the vase is extended
[49]
Gerbera ‘Ruikou’ (Gerbera jamesonii)(2-5 nm)  5 ppmAround the trunk for 24 hours
● Inhibits bacterial growth
● Absorption is maintained
● The life of the vase is extended
[50]
gladiolus

(Gladiolus hybridus)

(10, 25, 50 ppm )Pulsing for 24 h
● The life of the vase is extended
● Increase water absorption
● Reduce water loss
● Inhibition of bacteria is inhibited and biofilm formation on the cut surface is inhibited
[51]
Lily ‘Bouquet’ (Lilium orientalis)(5, 15, 25, 35 ppm)Add to the flower arrangement solution
● The vase prolongs the life of the flower
● Increase solution absorption
● Improved fresh weight
● Bacterial colonization is inhibited during the first two days of the flask life cycle
[52]
Lily ‘Shocking’ (Lilium orientalis)(5, 15, 25, 35 ppm)Adding to vase solutions
● The vase prolongs the life of the flower
● Increase solution absorption
● Improved fresh weight
● Bacterial colonization is inhibited during the first two days of the flask life cycle
[53]
Rosa ‘Tineke’ (Rosa hybrida)(1, 3, 5 ppm )Add to the flower arrangement solution
● Increase the life of the vase
● Improved water content
● Increase fresh weight
[54]
Hoa hồng ‘Avalanche’ and ‘Fiesta’ (Rosa)(50, 100, and 200 mg L− 1)Pulsing
● Increase the life of the vase
● Improved water absorption
● Increase fresh weight
● Reduce the amount of bacteria
● Minimized dehydration
● Increase the electrical conductivity of the stomata
● Improved steam evaporation rate
[55]
Rose‘Cherry Brandy’ (Rose)Nano-silver (1, 2.5 or 5%)Add to the flower arrangement solution● Increased vase life

● Improved solution uptake

● Retarded weight loss

● Disinfected vase solution

● Prevented vase solution microbial proliferation

[56]
‘First Red’ (Rose)(25, 50 and 100 ppm)Pulsing for 24 h
● The life of the vase is extended
● Inhibits growth of microorganisms
● Reduced ethylene production
[57]
 Rose ‘Movie Star’ (Rose)(0.5 and 10 ppm)The pulse for 24 hours and then is kept in a low concentration of NS solution
● Improve water balance
● The life of the vase is extended
● Increase flower weight
[58]
LiliesTuberose (Polianthus tuberosa)(15, 30 and 45 ppm)Add in preservative solutions
● Extend service life
● Increase the stem diameter
● Improved water absorption
● Increase fresh weight
● Relatively increases water absorption
[59]
 ‘Peril’

(Polianthes

tuberosa)

(0.5, 10 and 15 ppm)Add to the flower arrangement solution
● Increase the chlorophyll content
● Reduce electrolyte leakage
[60]
Tulip ‘White Parrot’ (Tulipa gesneriana)(10, 20 and 40 mg L-1 )Add to the flower arrangement solution
● Increase final fresh weight
● Improved initial fresh weight percentage
● The final body length is raised
● Increase the percentage of original body length
● Improved chlorophyll content
[61]

 

Conclusion
Our review shows that nano silver can be used in horticulture practice as a potential plant growth regulator. The effectiveness of nano-silver depends on the plant species, concentration, method of use, as well as the growing conditions and development stage. Therefore, we conclude that further research on the controversial or controversial issues and to focus on the unresolved issues associated with the use of nano-silver in plants is necessary.

Source: Nano-silver as a potential biostimulant for plant – A review

Department of Horticulture, Faculty of Environmental Management and Agriculture,
West Pomeranian University of Technology, Szczecin, Poland

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