Nano silver has antifungal properties on the skin Dermatophytes
Spherical silver nanoparticles (nano-Ag) were synthesized and their antifungal effects against skin pathogens were investigated. Nanosilver showed strong activity against clinical isolates and ATCC strains of Trichophyton mentagrophytes and Candida species (IC80, 1-7 µg/ml). The activity of nano-Ag was comparable to that of amphotericin B, but was superior to that of fluconazole (amphotericin B IC80, 1-5 µg/ml; fluconazole IC80, 10-30 µg/ml). In addition, we investigated their effect on Candida albicans dimorphism. The results showed that nano-Ag had an effect on mycelium. Therefore, the present study indicates that nanosilver may have significant antifungal activity, which deserves further investigation for clinical applications.
(Copyright by NanoCMM Technology)
Customers who need 15000 ppm nanosilver material for pharmaceutical use, please contact Hotline +84.0378.622.740 or +84.098.435.9664
Skin infections caused by fungi, such as Trichophyton and Candida species, have become more common in recent years . In particular, fungal infections are more common in patients who are immunocompromised due to cancer chemotherapy, or infected with human or organ immunodeficiency virus . This upward trend is worrisome, considering the limited number of antifungal drugs available because antifungal prophylaxis can lead to the emergence of resistant strains. Therefore, there is an urgent and unavoidable medical need for new antifungal drugs. Since ancient times, it has been known that silver and its compounds are effective antibacterial agents [6, 14, 15]. In particular, due to recent advances in metal nanoparticles research, nano-Ag has received special attention as a possible antimicrobial agent [1, 7, 9, 16]. Therefore, the preparation of uniform nano-sized silver particles with specific requirements on size, shape and physico-chemical properties is of great interest in new pharmaceutical formulations [3, 10]. . Many studies have shown their antibacterial effects, but the effect of nanosilver on pathogenic fungi on the skin is mostly unknown. In this study, nano-Ag was synthesized and its antifungal effect on clinical isolates and ATCC strains of Trichophyton mentagrophytes and Candida species were investigated.
Nano silver production
Determination of susceptibility to fungi
A total of 44 strains of 6 fungal species were used in this study. Candida albicans (ATCC 90028), Candida glabrata (ATCC 90030), Candida parapsilosis (ATCC 22019) and Candida krusei (ATCC 6258) were obtained from the American Type Culture Collection (ATCC) (Manassas, VA, U.S.A.). Clinical isolates of Candida spp. were obtained from the Department of Laboratory Medicine, Chonnam National University School of Medicine (Gwangju, Korea), and clinical isolates of Trichophyton mentagrophytes were obtained from the Institute of Medical Mycology, Catholic Dermatology Clinic (Daegu, Korea). South Korea). Candida spp. and Trichophyton mentagrophytes were cultured in Sabraud dextrose agar (SDA) and potato dextrose agar (PDA) at 35°C, respectively. MIC kit for Candida spp. and T. mentagrophytes were determined using a broth dilution method based on the method of the National Committee for Clinical Laboratory Standards (NCCLS; now renamed the Clinical and Laboratory Standards Institute, CLSI) , 2000) are outlined in documents M-27A  and M-38P , respectively. RPMI 1640 medium buffered to pH 7.0 with 3 propanesulfonic acid (N-morpholino) (MOPS) was used as the culture and size medium for Candida spp. is 0.5 × 103 to 2.5 × 103 cells/ml and that of T. mentagrophytes is 0.4 × 104 to 5 × 104 cells/ml. Inoculated microbiological dilutions were incubated at 35°C, and turbidity of the growth control wells was observed every 24 h. The 80% inhibitory concentration (IC80) was determined to be the lowest concentration that inhibits 80% of growth as determined by comparison with growth in control wells. Growth was examined using a microplate reader (Bio-Tek Instruments, Winooski, VT, U.S.A.) by monitoring the absorption at 405 nm. In the present study, amphotericin B and fluconazole were used as an active control for the fungus; amphotericin B is a fungicide widely used in the treatment of serious systemic infections , and fluconazole is used in the treatment of superficial skin infections caused by dermatophytes and Candida species . nanosilver, in the IC80 range from 1-7 µg/ml, showed significant antifungal activity against T. mentagrophytes and Candida species. For all fungal strains, nanosilver exhibited similar activity to amphotericin B, showing an IC80 value of 1-5 µg/ml. Nano silver showed itself to be more active than fluconazole, showing an IC80 value of 10-30 µg/ml. However, this compound exhibited less activity than amphotericin B, showing an IC80 value of 2-4 µg/ml for C. parapsilosis and C. krusei (Table 1)
Description: IC80 concentration of test solution inhibits 80% of fungi.
Reference: Antifungal effect of silver nanoparticles on dermatophytes
Kim, Keuk-Jun1, Woo Sang Sung1, Seok-Ki Moon2, Jong-Soo Choi2, Jong Guk Kim1,
and Dong Gun Lee1*