Nano silver is used to preserve and treat diseases in agriculture
In general, the present invention relates to colloidal silver nanoparticles, and more specifically, substances and methods for the preservation and treatment of diseases in the agricultural field.
(Copyright by NanoCMM Technology)
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THE BACKGROUND OF THE INITIALITY
0002 Pests, weeds, insects, molds, bacteria and other harmful agents that adversely affect the health and development of agricultural products. Considerable effort has been made to develop organic chemicals that selectively prevent, reduce or prevent damage caused by these pathogens to agriculture. However, chemicals also tend to enter ecosystems and cause undesirable environmental and environmental effects. Desired technologies can reduce the use of organic chemicals while ensuring that the harmful effects of pests, weeds and other agents are checked or prevented. Another limitation of current technology based on the use of organic chemicals is the use of ineffective dosages. Usually, the concentration of the dose when it is first applied is very high and then decreases rapidly. Such an application provides more than the desired concentration immediately after application and then falls too low from the desired concentration several days after application. In many cases, the protection is more uniform and continuous from pests, weeds, etc. prioritized. Current technologies cannot provide this.
In one embodiment, the present invention provides a simple preparation method for silver nanoparticles of well controlled size in a surfactant solution. Nano silver colloid is prepared by the following steps – (1) dissolve the silver nitrate crystal in distilled water, (2) add the surfactant, LABS (Linear alkylbenzene sulfonate) to the solution and; (3) add reducing agent to the solution.
0004 In addition, the present invention provides methods for using nano-silver colloids for the preservation and treatment of diseases in the agricultural field.
In the preferred embodiment, the present invention describes processes and products for the preservation and treatment of diseases in the agricultural sector. The present invention describes technologies for activating silver nanomaterials to improve agricultural quality, increase shelf life and quality of agricultural products, preserve and treat agricultural microbial diseases, reduce kill microorganisms present on seeds or kill pathogens by using seeds for subsistence, soil and greenhouse disinfection, and development and growth of roots and leaves in their number and size.
BRIEF DESCRIPTION OF DRAWINGS
The invention, as well as its many advantages, can be better understood by the following detailed description and drawings:
0007 PICTURE. 1 shows the UV absorbance of porous beads prepared in the presence of different Surfactants.
0008 PICTURE. 2(A) to 2(C) show the particle size distribution of silver particles prepared in different surfactants.
0009 PICTURE. 2(A) shows LABS, which is an anionic surfactant.
0010 PICTURE. 2 (B) shows SDS, which is an anionic surfactant.
0011 PICTURE. 2 (C) shows Tween 20, which is a non-ionic surfactant.
According to an embodiment, the invention proposed
Preparation of metal nanoparticles by using anionic and non-ionic surfactants and suitable metal salts and reducing agents, metal salts can be effectively reduced to metal nanoparticles with uniform particle size. A suitable surfactant useful in the process of the present invention is LABS (straight chain alkylbenzene sulfonate), tween 20 (polysorbate 20). Tween 60 (polysorbate 60), Tween 80 (polysorbate 80), SDS (sodium dodecyl sulfate). That’s the best outcome for LABS. In general, the amount or concentration of Surfactant used in the present invention may be, but is not limited to, 0.05-20% by weight, preferably; amount is 2-5 wt%.
0013 Suitable reducing agents useful in the process under the present invention are hydrazine, NaBH, LiBH’, Glucose. The best result was hydrazine. In general, the amount or concentration of hydrazine used in the present invention may be, but is not limited to, 1×10-510g. Best, the quantity is 3×10-5x10g.
This silver nanocolloid was prepared at room temperature and with a reaction time of 1 hour, and it was possible to synthesize this colloid in less time.
0015 The process for preparing metal nanoparticles according to the present invention exhibits the following advantages:
0016 Controlling the size of nanoparticles with a simple method.
0017 Easy to use and requires no specific expertise.
(0018 Wide application.
(0019 Minimal side effects on the body.
It is a strong insecticide against bacteria.
0021 Stable compounds.
The smallest particles (nano).
(0023. Short synthesis time.
Use water solvents instead of organic solvents.
The process of the invention described herein, with reference to the following examples, is for illustrative purposes only and should not be construed as limiting the scope of the invention.
4.5 g LABS (linear alkylbenzene sulfonate) was dissolved in 95 ml of distilled water and then added to a solution containing 0.32 g of silver nitrate. After thorough mixing, 0.2 g of hydrazine solution (0.03 M) was added to the solution, a yellow colloidal silver solution was formed. The UV-Vis spectrum of the reaction solution shows an absorption bond at about 415 nm which is the characteristic absorption bond of silver nanoparticles. Dynamic light scattering results of the Solution reaction, as shown in Fig. 2 (A), showing an average grain size of 4.5 nm.
The same procedure as in example 1 was repeated except that 4.5 g LABS was replaced with 4.5 g SDS (sodium dodecyle sulfate). The UV spectrum of the colloidal silver nanoparticle solution was obtained in Figure 1, and the average particle size was 78.2 nm, as shown in Fig. 2 (B).
Tween 20 (polysorbate 20) was used instead of LABS in Example 1. UV spectra of the colloidal silver solution obtained in Fig. 1 and the average particle size is 32.5 nm, as shown in Fig. 2 C).
Colloidal silver nanoparticles prepared in examples 1-3 were examined to determine the antibacterial activity of the colloid.
0030 Provide sterile NA culture medium and its purification plate.
0031 Supply of microbiological suspension of E-coli standard Soosh
0033 according to the McFarland method that it has a concentration of 158 cfu/ml.
0034 Provide a microbiological suspension with a concentration of 105 from the above suspension.
0035 Mix / 3 nano colloids and 2/3 of the bacteria provided.
0036 Culture the blend material in the previous step on NA culture medium.
0037 Place the plates in the incubator at 25°C for 24 h.
After 24 h, a loop was taken from those plates and placed on fresh NA medium.
After 24 h in the incubator, the plates were examined for bacterial colony counts.
The revealed solution has been used to control sepia leaf spot, yellow rust, edema and powdery mildew in wheat, showing that the nanoscanner controls wheat diseases. At low doses of nanosilver, nanosilver controlled schlorotenia in rapeseed or rapeseed and sphaerotheca foliginea in cucumber and rapeseed and rose seeds, and leaf curl disease in peach orchards.
0041 After harvesting for extra shelf life, we used nanofilters to prevent and control any microorganisms that cause problems and can damage our products. The solution has been tested on different agricultural products such as citrus, lime, wheat, barley and the solution can prevent their damage. Furthermore, nanosilver active materials have been used to package agricultural products. The results were as follows: damage control in the package and shelf life more than control.
Experiments demonstrate that the invention improves growth stimulation in all cases. When seeds were treated with nano silver active ingredient, the seeds grew at a high rate and had longer roots. The active substance is sprayed on the plant, which changes the size of the leaves and makes the leaves fresher.
A solution is made with several doses of nanosilver, and then the seeds are treated to check their germination. In the case of wheat, the following are references. 0ppm, 20 ppm, 40 ppm, 60 ppm, 80 ppm with nano silver and seed treatment (planted on sand under standard conditions, after a week, the following results are obtained: seeds planted at 0 ppm, 70%; in 20 ppm we have 78 out of 100, in 40 ppm % 90, in 60 ppm % 96 and 80 ppm % 90 in germination. tested in 20 ppm 12%, in 40 ppm 26%, 60 ppm 18% and 80 ppm 4%.This test shows that with the active material of the invention there is not only an increase in the percentage of growth. but also acceleration in the growing particle.
0044) The test was performed on wheat on the farm. Same results as above and moreover, seed planting has been improved.
Testing was done on cabbage seeds with similar results as above. That is, at 0 ppm or the control there was 69% germination, at 20 ppm it was 77%, at 40 ppm it was 86% and at 60 ppm it was 78%. These tests were repeated on corn and cotton and many other grains, and the results were similar.
The above tests were performed on the shoots of olive trees by dipping the Scissors in Nano Silver Solution and planting them in the sand located inside the green house, the root stimulation that occurs in the Olive Scion, as well as the olive scion. Nano silver solution has preserved the disease resistant branches in the fog.
By spraying the solution on strawberries to serve the farm, the strawberries never got sick and compared to the control, the result was bigger fruit, bigger leaves, and the disease was treated.
Further tests carried out on the wheat farm were associated with numerous diseases such as Erysiphe graminis, septoria and fitarium. A comparison was made between the Patent Solution and the other method. On some insecticides, after calculating the distribution of the disease on leaves after 10 days, after 20 and 30 days, the active ingredient nono-silver was ranked first.
The test was done on apple tree erwinia amilovora, which is the bacteria that causes witch disease. Not only controlled this disease, but also obtained larger and glossy leaves than the control.
The test was carried out on maize and rice cultivation in the same way as it was done on wheat and as a result toxicity was eliminated.
The solution is tested on an orange. When dipping oranges in the solution, the shelf life of the fruit was increased for a longer time, e.g. with 10 ppm about 2 grass compared to the control and with 50 ppm of the silver nano solution more than 2 months compared to the control. .
0052 For Nano silver packaging, the Nano silver active material is mixed with the polymer, so the packaging has a longer shelf life.
The test was carried out on the packaging even on milk and meat, fruit and dry goods, the results were similar.
0054) Other variants of the invention will be obvious to those skilled in this field considering the specification or practice of the invention disclosed herein. The purpose of the specification and the examples are to be regarded as exemplary only, with the true scope and spirit of the invention indicated by the following requirements.
What is declared:
Nano silver active ingredient for agricultural products, this nano silver active ingredient includes: Colloidal silver, silver powder, silver composites, silver compounds and silver salts, in which the aforementioned silver salts absorb silver nitrate and silver chloride.
Silver nanoactive material according to point 1, where the active silver nanomaterial has a diameter between 1 and 250 nanometers, and where the active material destroys microorganisms and pathogens.
Silver nano-active material according to point 1, where the active material coats the seed and prevents pathogens from attacking the seed, thus improving the growth of said seed.
Nanosilver active materials according to point 1, in which this active material increases the shelf life of said particles during storage and transportation of said particles.
Nanosilver active material according to point 1, where the active material is applied to vegetable soil and green house bed, where this green house substrate includes hydroponic system and/or Iroponic system.
Nano silver active ingredient according to point 1, where it is applied to spring onions, onions, Pruning, cutting and seeds, thus disinfecting the aforementioned onions, seeds and Pruning against diseases in soil and beds Greenhouse and destroy any pathogens on/in of the aforementioned onions, seeds and Scions.
Nano silver active ingredient according to point 1, in which this active substance stimulates the growth of roots and leaves, and increases the size and number of the aforementioned roots and leaves.
Nanosilver active material according to point 6, where this active material accelerates root growth in the aforementioned Scions, onions and seeds.
Silver nanoactive material according to point 1, where this active material increases light absorption, thus increasing photosynthesis.
Nano silver active material according to point 1, where this active material is applied for packaging of agricultural products, thus increasing the shelf life of this product.
Nano silver active ingredient according to point 1, where it is used on cut flowers and agricultural products after cutting and harvesting, on farms, in orchards and in green houses in spray or dipping, thereby increasing the shelf life and improving the quality of cut flowers and agricultural products mentioned above.
Nano silver active ingredient according to point 1, where it is used during storage, through direct spraying on crops, irrigation or injection into plants and crops, thus preventing and treating diseases in farms, orchards and greenhouses.
Reference: US 2009/0075818 A1